Most retroviral recombinations occur during minus-strand DNA synthesis.

@article{Zhang2000MostRR,
  title={Most retroviral recombinations occur during minus-strand DNA synthesis.},
  author={Jiayou Zhang and Linda Y F Tang and T Li and Yinghong Ma and C M Sapp},
  journal={Journal of virology},
  year={2000},
  volume={74 5},
  pages={
          2313-22
        }
}
Retroviral RNA molecules are plus, or sense in polarity, equivalent to mRNA. During reverse transcription, the first strand of the DNA molecule synthesized is minus-strand DNA. After the minus strand is polymerized, the plus-strand DNA is synthesized using the minus-strand DNA as the template. In this study, a helper cell line that contains two proviruses with two different mutated gfp genes was constructed. Recombination between the two frameshift mutant genes resulted in a functional gfp. If… CONTINUE READING

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