Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes

@article{Almoguera1988MostHC,
  title={Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes},
  author={C. Almoguera and D. Shibata and K. Forrester and J. Martin and M. Perucho},
  journal={Cell},
  year={1988},
  volume={53},
  pages={549-554}
}
Using in vitro gene amplification by the polymerase chain reaction (PCR) and mutation detection by the RNAase A mismatch cleavage method, we have examined c-K-ras genes in human pancreatic carcinomas. We used frozen tumor specimens and single 5 micron sections from formalin-fixed, paraffin-embedded tumor tissue surgically removed or obtained at autopsy. Twenty-one out of 22 carcinomas of the exocrine pancreas contained c-K-ras genes with mutations at codon 12. In seven cases tested, the… Expand
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References

SHOWING 1-10 OF 21 REFERENCES
Activation of the c-K-ras oncogene in a human pancreas carcinoma.
  • 44
The ras gene family and human carcinogenesis.
  • J. L. Bos
  • Biology, Medicine
  • Mutation research
  • 1988
  • 750
Activation of a human c-K-ras oncogene.
  • 32
Prevalence of ras gene mutations in human colorectal cancers
  • 1,817
A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells.
  • 293
  • PDF
Isolation of transforming sequences of two human lung carcinomas: structural and functional analysis of the activated c-K-ras oncogenes.
  • 108
  • PDF
Pancreatic neoplasia induced by ras expression in acinar cells of transgenic mice
  • 249
Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction
  • 693
  • PDF
Detection of high incidence of K-ras oncogenes during human colon tumorigenesis
  • 1,047
...
1
2
3
...