Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus.

@article{Kanthong2008MosquitoCA,
  title={Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus.},
  author={N. Kanthong and Nuanpan Khemnu and S. Sriurairatana and S. Pattanakitsakul and P. Malasit and T. Flegel},
  journal={Developmental and comparative immunology},
  year={2008},
  volume={32 9},
  pages={
          1063-75
        }
}
Key MethodTo study persistent viral co-infections in arthropods, we first produced stable, persistently infected C6/36 mosquito cell cultures by serial passage of exponentially growing whole cells infected with either a densovirus (AalDNV) or Dengue virus (DEN-2). We then obtained stable, persistent co-infections by reciprocal super-challenge and similar passaging. Persistently infected cultures did not differ from naïve-cell cultures in growth rate and cell morphology. Nor did they differ in high…Expand
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References

SHOWING 1-10 OF 45 REFERENCES
Replication of Dengue Virus Type 2 in Aedes albopictus Cell Culture
TLDR
The replication of type 2 dengue (D-2) virus in Aedes albopictus (Aal) mosquito cell cultures differed from that in vertebrate (LLC-MK2) rhesus monkey kidney cells, suggesting a difference in the mechanism by which D-2 virus matures in Aal cells. Expand
Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus.
TLDR
Three Aedes albopictus (mosquito) cell lines persistently infected with Sindbis virus excluded the replication of both homologous and heterologous alphaviruses, indicating that the expression of superinfection exclusion is conserved among A. albipictus cell types. Expand
Susceptibility of the Sf9 insect cell line to infection with adventitious viruses.
TLDR
It is demonstrated using double immunofluorescence that single cells are dually infected with SLE virus and rBV, and several viruses which are pathogenic for humans are stable for long periods of time in the serum-containing media used for cultivation of Sf9 cells. Expand
Markedly reduced severity of Dengue virus infection in mosquito cell cultures persistently infected with Aedes albopictus densovirus (AalDNV).
TLDR
Questions are raised regarding the potential for persistent infections by unknown viruses to confound experimental results with insect cell lines and reasons for rapid reduction in APC and resistance to superinfection upon serial passage remain unproven. Expand
Challenging successive mosquito generations with a densonucleosis virus yields progressive survival improvement but persistent, innocuous infections.
TLDR
It is possible that the presence of prior AThDNV infections with a high level of DIP contributed to improved survival in the challenged F4 mosquito population, and suggests that persistent viral infections in arthropods may serve in a specific, adaptive manner to reduce the incidence and severity of disease. Expand
Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line.
TLDR
There does not seem to be an antiviral state induced by AeD NV persistent infection, and C6/36 cells persistently infected with AeDNV were not protected from superinfection with HeDNV. Expand
Persistence of Japanese encephalitis virus is associated with abnormal expression of the nonstructural protein NS1 in host cells.
TLDR
Data indicate a strong association between abnormal NS1 expression and JEV persistence in cell culture and a probable involvement of dysfunctional NS1 in the establishment and/or maintenance of JEV persistency in tissue culture. Expand
Densoviruses for control and genetic manipulation of mosquitoes.
TLDR
The availability of infectious clones of MDV genomes allows the development of densovirus vectors for expressing genes of interest in mosquito cells and mosquitoes and recently short hairpin RNA expression cassettes that induce RNA interference have been inserted into densov virus genomes. Expand
E/NS1 Modifications of Dengue 2 Virus after Serial Passages in Mammalian and/or Mosquito Cells
TLDR
It is concluded that genetic changes of dengue 2 virus are constrained in Vero (mammalian) cells, resulting in a variety of genome-related quasispecies populations. Expand
Dual Mechanisms of Pestiviral Superinfection Exclusion at Entry and RNA Replication
TLDR
Findings suggest dual mechanisms of pestivirus superinfection exclusion, one at the level of viral entry that requires viral glycoprotein E2 and a second at thelevel of viral RNA replication. Expand
...
1
2
3
4
5
...