• Corpus ID: 244714704

Morphomics via Next-generation Electron Microscopy

  title={Morphomics via Next-generation Electron Microscopy},
  author={Raku Son and Kenji Yamazawa and Akiko Oguchi and Mitsuo Suga and Masaru Tamura and Yasuhiro Murakawa and Satoshi Kume},
Affiliations 1. RIKEN-IFOM Joint Laboratory for Cancer Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan 2. Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, Japan 3. Advanced Manufacturing Support Team, RIKEN Center for Advanced Photonics, Wako, Japan 4. Multi-Modal Microstructure Analysis Unit, RIKEN-JEOL Collaboration Center, Kobe, Japan 5. Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Research Center… 

Figures from this paper



The 2018 correlative microscopy techniques roadmap

A special roadmap on the correlative microscopy techniques is presented, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints.

TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages

Evaluation of the applications and advantages of each method highlights their practicality for the identification, quantification, and characterization of heterogeneous cell populations in small organisms, organs, or tissues in healthy and diseased states.

Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects

A simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy and makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels.

Transmission electron microscopy in cell biology: sample preparation techniques and image information

This chapter aims to provide an overview of the most important techniques that one can apply to a biological sample, tissue or cells, to observe it with an electron microscope, from the most conventional to the latest generation.

Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells

This protocol provides guidelines for designing and validating APEX2 fusion constructs, along with detailed instructions for cell culture, transfection, fixation, heavy-metal staining, embedding in resin, and EM imaging, and is useful for numerous applications beyond EM.

Structural Stabilization of Tissue for Embryo Phenotyping Using Micro-CT with Iodine Staining

Hydrogel stabilization substantially reduces intersample anatomical variation of mature mouse embryos subjected to iodine preparation protocols and increases the sensitivity of computer automated analysis to reveal significant anatomical differences between mutant and wild-type mice.

Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography

A new subcellular compartment of podocytes, “ridge-like prominences” (RLPs) is identified, which allows for the 3D reconstruction of podocyte ultrastructure, which can be viewed from all angles, from a single image set, not possible with conventional FE-SEM.

Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy

A novel correlative workflow combining intravital microscopy, microscopic X-ray computed tomography and 3D electron microscopy to reveal metastatic events in mouse brain and skin tissue at high resolution is provided.

Informative three-dimensional survey of cell/tissue architectures in thick paraffin sections by simple low-vacuum scanning electron microscopy

A three-dimensional survey method for assessing cell/tissue architectures in 30-µm-thick paraffin sections by taking advantage of backscattered electron imaging in a low-vacuum scanning electron microscope is developed and representative electron micrographs of normal rat organs, experimental thrombus formation, and three-dimensionally cultured tumour cells are provided.

Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon

A comprehensive overview of the field of CMI from preclinical hybrid imaging to correlative microscopy is presented, requirements for optimization and standardization are highlighted, and current efforts to bridge the gap between preclinical and biological imaging are focused on.