Morphology of hepatitis C and hepatitis B virus particles as detected by immunogold electron microscopy

@article{Kaito2006MorphologyOH,
  title={Morphology of hepatitis C and hepatitis B virus particles as detected by immunogold electron microscopy},
  author={Masahiko Kaito and Satoshi Ishida and Hideaki Tanaka and Shinichiro Horiike and Naoki Fujita and Yukihiko Adachi and Michinori Kohara and Masayoshi Konishi and Shozo Watanabe},
  journal={Medical Molecular Morphology},
  year={2006},
  volume={39},
  pages={63-71}
}
We performed indirect immunogold electron microscopy (EM) for immunological identification and characterization of hepatitis C virus (HCV). To clarify the morphology of HCV, an indirect immunogold EM of two plasma samples from patients with high HCV RNA titers was carried out using antibodies specific for the putative HCV envelope protein (E) 1. Spherical virus particles 55–65 nm in diameter with delicate spike projections were detected in the 1.14–1.16 g/ml fractions after sucrose density… 
The ultrastructural morphology of native hepatitis B virus
TLDR
It is shown for the first time that the native replicative form of HBV is cobra- and horn-shaped, which is significantly different from that of unpurified samples.
Unidentified virus-like particles are detected in plasmas with elevated ALT levels: are they significant of etiological agent(s) of non-B, non-C hepatitis?
TLDR
Spherical VLPs are detected in HBV- and HCV-negative plasmas significantly correlated with the elevation of ALT, suggesting that they are implicated in non-B, non-C hepatitis.
Cellular targets and pathways of yellow head virus infection in lymphoid organ of Penaeus monodon as studied by transmission electron microscopy
TLDR
Negative-stained intact yellow head virus (YHV) was an enveloped bacilliform particle measuring 40-50 x 175-210 nm with spike-like projections measuring 7-9 nm, and the RNA genome had 40- 50 turns in a helical structure.
Hepatitis C virus core antigen: analytical performances, correlation with viremia and potential applications of a quantitative, automated immunoassay.
  • M. Medici, G. Furlini, C. Chezzi
  • Medicine, Biology
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
  • 2011
Lack of infectivity of HBV in feces from patients with chronic hepatitis B virus infection, and infection using chimeric mice
TLDR
Feces from HBV carriers seem not to serve as an infectious vehicle for the transmission of HBV, and chimeric mice were not infected with HBV after oral or intravenous inoculation with sterilized fecal samples.
Detection of RNA viruses from influenza and HIV to Ebola and SARS-CoV-2: a review.
TLDR
The detection of the viruses (influenza, hepatitis, HIV, Zika, Sars, Ebola, SARS-CoV-2, etc.) and human antibodies to these viruses is described and tabulated in terms of the reported methods of detection, time to results, accuracy and specificity, if they are reported.
Hepatitis Viruses as Sexually Transmitted Diseases
TLDR
This chapter will cover each hepatitis virus, discussing epidemiology, viral structure, genome and life cycle, pathogenesis, treatment, and complications.
Prevalence of Hepatitis C virus Genotype among Hemodialysis Patients Two
TLDR
Overall Hepatitis C virus genotypes prevalence among hemodialysis patients is similar to those in most of the peripheral countriesto Iraq, but at the local level, some he modialysis centers that have prevalent genotypes different from those in the general population may be found.
Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis
TLDR
This study identified 22 differentially expressed proteins related to liver fibrosis, and verified a potential biomarker (ANXA2) for non‐invasive diagnosis of immune Liver fibrosis diagnosis through PM proteome research.
Recent Electrochemical Methods Proposed for the Detection of Hepatitis C Virus. A Minireview
TLDR
This review is summarizing new methods of detection of specific biomarkers/genotypes for viral hepatitis C, to help find the highest sensitive screening methods that may influence the decision of the most efficient treatment.
...
1
2
...

References

SHOWING 1-10 OF 22 REFERENCES
Hepatitis C virus core particle detected by immunoelectron microscopy and optical rotation technique.
Hepatitis C virus particle detected by immunoelectron microscopic study.
To clarify the morphology of hepatitis C virus (HCV), an indirect immunogold electron microscopic study was carried out on two plasma samples with high HCV RNA titres using polyclonal and monoclonal
Paraformaldehyde protects of hepatitis C virus particles during ultracentrifugation
TLDR
A useful method for the purification of HCV virions is described, and new insights for elucidating the physicochemical properties ofHCV particles are provided.
Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction
TLDR
The results suggest that quantitation of HCV RNA may be useful not only for understanding the course ofHCV infection but also for evaluating treatment for HCV infection.
Structure and organization of the hepatitis C virus genome isolated from human carriers
TLDR
The hydrophobicity profile of the putative polyprotein is similar to those of flaviviruses, but it has limited amino acid homology to polyproteins of flavIViruses and other viruses, indicating that HCV is at most distantly related to flavivIRuses.
Quantitation of Hepatitis B Virus Genomic DNA by Real-Time Detection PCR
TLDR
This work attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems and found that RTD-PCR is an excellent candidate for a standard HBV quantification method.
Detection of hepatitis C virus by polymerase chain reaction and response to interferon‐α therapy: Relationship to genotypes of hepatitis C virus
TLDR
Results indicate that detection of hepatitis C virus RNA by polymerase chain reactions with different sets of primers and probes may be valuable in classifying hepatitis Cirus into genotypes, and that amount of hepatitisC virus RNA in sera and response to interferon‐α may vary among different genotypes of HCV.
Hepatitis A: Detection by Immune Electron Microscopy of a Viruslike Antigen Associated with Acute Illness
TLDR
Spherical 27-nanometer particles were visualized in stools obtained from hepatitis A patients in the acute phase of the disease and suggest that it is the etiologic agent of hepatitis A.
Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese
...
1
2
3
...