Morphological changes of in-vitro-produced bovine blastocysts after vitrification, in-straw direct rehydration, and culture.

@article{Vajta1997MorphologicalCO,
  title={Morphological changes of in-vitro-produced bovine blastocysts after vitrification, in-straw direct rehydration, and culture.},
  author={G{\'a}bor Vajta and Poul Hyttel and Henrik Callesen},
  journal={Molecular reproduction and development},
  year={1997},
  volume={48 1},
  pages={
          9-17
        }
}
  • Gábor Vajta, Poul Hyttel, Henrik Callesen
  • Published in
    Molecular reproduction and…
    1997
  • Biology, Medicine
  • Morphological signs of injury and regeneration following vitrification and warming of bovine embryos were studied by light and electron microscopy. In-vitro-produced Day 7 expanded blastocysts (Day 0 = day of insemination) were vitrified by a two-step equilibration method using ethylene glycol and dimethyl sulphoxide as cryoprotectants. Thawing was performed by in-straw direct rehydration, followed by in vitro culture on a granulosa cell monolayer. Embryos were processed for transmission… CONTINUE READING

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