Morphological changes of dermal fibroblasts of rat following transfection with MyoD cDNA in vitro

@inproceedings{Yi2007MorphologicalCO,
  title={Morphological changes of dermal fibroblasts of rat following transfection with MyoD cDNA in vitro},
  author={Din Yi},
  year={2007}
}
Objective To evaluate the possibility of converting cultured rat dermal fibroblasts(DFs) to skeletal muscle cells by transfection with myoblast-determining gene(MyoD cDNA),one of the basic helix-loop-helix myogenic factors.Methods DFs of Sprague-Dawley(SD) rat were isolated and cultured with an improved technique of differential anchoring velocity;MyoD cDNA was transduced into DFs by using the eukaryotic expression vector pLenti6/V5-DEST-MyoD,and the cells were incubated in medium containing… CONTINUE READING