Morphological changes in ascospores of Saccharomyces cerevisiae during aerobic and anaerobic germination.

@article{Sando1980MorphologicalCI,
  title={Morphological changes in ascospores of Saccharomyces cerevisiae during aerobic and anaerobic germination.},
  author={Nobundo Sando and Tomoko Oguchi and Misuzu Nagano and Masako Osumi},
  journal={Journal of General and Applied Microbiology},
  year={1980},
  volume={26},
  pages={403-412}
}
The stainability of ascospores and vegetative cells of Saccharomyces cerevisiae to acid-fast staining, using hot Ziehl's carbolic fuchsin solution, 5% sulfuric acid, and diluted Loffler's methylene blue, was examined. Resting spores and growing haploid cells (a type strain 24428 and α type 3626) retained much fuchsin dye in the cells. Only mature spores of diploid G2-2 resisted methylene blue staining. The stainability of Mycobacterium phlei IFO 3158 also examined.The kinetics of germinationn… 
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