Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action

  title={Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action},
  author={Tim Cushnie and Noelle H O'Driscoll and Andrew J. Lamb},
  journal={Cellular and Molecular Life Sciences},
Efforts to reduce the global burden of bacterial disease and contend with escalating bacterial resistance are spurring innovation in antibacterial drug and biocide development and related technologies such as photodynamic therapy and photochemical disinfection. Elucidation of the mechanism of action of these new agents and processes can greatly facilitate their development, but it is a complex endeavour. One strategy that has been popular for many years, and which is garnering increasing… 

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Scanning electron microscopy of changes in bacteria induced by antimicrobial agents which interfere with cell wall synthesis revealed morphological alterations which correlated well with their

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Bacterial swelling with scarcity and vacuole-formation of the cytoplasm characterized the majority of the staphylococci during thePAE and the increased metabolic activity, coincident with increased duration of the PAE, suggests a possible common mechanism.

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The results demonstrate that both TEM and SEM, as well as appropriate sample preparation protocols, are needed to obtain detailed mechanistic insights into peptide function.

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