Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy

@article{Gjerde2017MorphologicalAM,
  title={Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy},
  author={Bj{\o}rn Gjerde and Stefano Giacomelli and Alessandro Bianchi and Irene Bertoletti and Hajime Mondani and Lucia Rita Gibelli},
  journal={Parasitology Research},
  year={2017},
  volume={116},
  pages={1317-1338}
}
Fresh (frozen/thawed) muscle samples from four 2–12-year-old roe deer (Capreolus capreolus) from the Sondrio province in north-eastern Italy were examined under a dissecting microscope, and about 180 sarcocysts were isolated and identified to morphological type in wet mounts by light microscopy (LM). Seventy-seven of these sarcocysts were subsequently examined by molecular methods, comprising polymerase chain reaction (PCR) amplification and sequencing of the partial cytochrome c oxidase… 
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References

SHOWING 1-10 OF 77 REFERENCES
Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway
TLDR
DNA derived from numerous cysts of S. gracilis harboured numerous oocysts, measuring about 20 × 15 μm, in their intestinal mucosa upon euthanasia 14 days post-inoculation, confirming for the first time by molecular methods that foxes are definitive hosts for this species.
Morphological and molecular characteristics of four Sarcocystis spp. in Canadian moose (Alces alces), including Sarcocystis taeniata n. sp.
Individual sarcocysts were isolated from fresh or alcohol-fixed muscle samples of two moose from Alberta, Canada, and examined by light (LM) and scanning electron microscopy (SEM) and molecular
Molecular characterisation of Sarcocystis lutrae n. sp. and Toxoplasma gondii from the musculature of two Eurasian otters (Lutra lutra) in Norway
TLDR
Sequence comparisons revealed that both otters were infected by the same Sarcocystis sp.
Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis)
TLDR
About 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology, and the utility of these regions for species delimitations and phylogenetic inferences was evaluated.
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania
TLDR
The phylogenetic analyses based on 18S rDNA and cox1 sequences suggest that canids are definitive hosts of both S. taeniata and S. pilosa, the first identification of Sarcocystis species in the sika deer by morphological and molecular methods.
Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species
TLDR
Fresh muscle tissue from six roe deer from Southeastern Norway was examined for sarcocysts andylogenetic analyses based on complete ssu rRNA gene sequences revealed a close relationship between S. gracilis and other canine-transmitted Sarcocystis species, whereas S. oviformis formed a well-supported group with Sarccystis hardangeri of reindeer and Sarcocrystis ovalis of moose.
Muscular sarcocystosis in two arctic foxes (Vulpes lagopus) due to Sarcocystis arctica n. sp.: sarcocyst morphology, molecular characteristics and phylogeny
TLDR
Sequence comparisons and phylogenetic analyses revealed that the species described from the two arctic foxes was distinct from other named Sarcocystis spp.
Sarcocystis entzerothi n. sp. from the European roe deer (Capreolus capreolus)
TLDR
Having examined six roe deer hunted in Lithuania, cysts of S. entzerothi and S. silva were identified in four and two animals, respectively, and these two Sarcocystis species could be morphologically differentiated according to the shape of the cysts and the length of protrusions.
Molecular differentiation of Sarcocystis buffalonis and Sarcocystis levinei in water buffaloes (Bubalus bubalis) from Sarcocystis hirsuta and Sarcocystis cruzi in cattle (Bos taurus)
TLDR
The phylogenetic analyses placed with high support the cox1 sequences of S. buffalonis and S. levinei from water buffaloes with similar sequences deposited in GenBank suggested that the two species were genetically different from S. hirsuta into two monophyletic sister groups.
A Light Microscopic Comparison of The Cysts of Four Species of Sarcocystis Infecting The Domestic Reindeer (Rangifer Tarandus) in Northern Norway
  • B. Gjerde
  • Biology, Medicine
    Acta Veterinaria Scandinavica
  • 1984
TLDR
Fresh preparations of micro-isolated sarcocysts from skeletal and cardiac muscle of 12 reindeer were examined by light microscopy and 2 previously unnamed Sarcocystis spp.
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