Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy

  title={Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy},
  author={Bj{\o}rn Gjerde and Stefano Giacomelli and Alessandro Bianchi and Irene Bertoletti and Hajime Mondani and Lucia Rita Gibelli},
  journal={Parasitology Research},
Fresh (frozen/thawed) muscle samples from four 2–12-year-old roe deer (Capreolus capreolus) from the Sondrio province in north-eastern Italy were examined under a dissecting microscope, and about 180 sarcocysts were isolated and identified to morphological type in wet mounts by light microscopy (LM). Seventy-seven of these sarcocysts were subsequently examined by molecular methods, comprising polymerase chain reaction (PCR) amplification and sequencing of the partial cytochrome c oxidase… 
Morphological and molecular characteristics of six Sarcocystis spp. from red deer (Cervus elaphus) in Spain, including Sarcocystis cervicanis and three new species
Samples of muscle tissue from the diaphragm, oesophagus and/or heart of eight adult red deer from the Quintos de Mora Park in Toledo, Central Spain, were screened for sarcocysts by means of the compression method, revealing the presence of six major cox1 sequence types.
Prevalence and molecular characterisation of Sarcocystis miescheriana and Sarcocystis suihominis in wild boars (Sus scrofa) in Italy
Phylogeny based on cox1 sequences placed S. miescheriana and S. suihominis as sister species within a clade comprising mainly Sarcocystis spp.
Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain
The microscopic sarcocysts belonged to a Sarcocystis mihoensis-like species with slanting thorn-like cyst wall protrusions, which was characterised molecularly for the first time and probably uses corvids as definitive hosts.
Morphological and molecular identification of Sarcocystis sp. from the little grebe, Tachybaptus ruficollis (Aves: Podicipediformes), for the first time in Egypt
This study is the first to report the occurrence of Sarcocystis sp.
Genetic characterization and phylogenetic analysis of Sarcocystis suihominis infecting domestic pigs (Sus scrofa) in India
The current study established the phylogenetic relationship of Indian isolates of S. suihominis with various Sarcocystis spp.
First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt
The present study is the first collective ultrastructural and molecular study that confirmed the taxonomy of sarcocysts isolated from water buffaloes in Egypt as Sarcocystis fusiformis.
Molecular differentiation of five Sarcocystis species in sika deer (Cervus nippon centralis) in Japan based on mitochondrial cytochrome c oxidase subunit I gene (cox1) sequences
This study characterized sarcocyst isolates from sika deer at the mitochondrial cytochrome c oxidase subunit I gene (cox1) and developed a multiplex PCR based on cox1 sequences of all species found, so that this assay may be useful for a rapid identification of sarcocysts of these species.
Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence
The current data showed that the present species was identified as S. fayeri and deposited in GenBank and highlighted the importance of the genetic data in the exact taxonomy within sarcocystid species.


Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway
DNA derived from numerous cysts of S. gracilis harboured numerous oocysts, measuring about 20 × 15 μm, in their intestinal mucosa upon euthanasia 14 days post-inoculation, confirming for the first time by molecular methods that foxes are definitive hosts for this species.
Morphological and molecular characteristics of four Sarcocystis spp. in Canadian moose (Alces alces), including Sarcocystis taeniata n. sp.
Individual sarcocysts were isolated from fresh or alcohol-fixed muscle samples of two moose from Alberta, Canada, and examined by light (LM) and scanning electron microscopy (SEM) and molecular
Molecular characterisation of Sarcocystis lutrae n. sp. and Toxoplasma gondii from the musculature of two Eurasian otters (Lutra lutra) in Norway
Sequence comparisons revealed that both otters were infected by the same Sarcocystis sp.
Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis)
About 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology, and the utility of these regions for species delimitations and phylogenetic inferences was evaluated.
Morphological and molecular characterization of Sarcocystis taeniata and Sarcocystis pilosa n. sp. from the sika deer (Cervus nippon) in Lithuania
The phylogenetic analyses based on 18S rDNA and cox1 sequences suggest that canids are definitive hosts of both S. taeniata and S. pilosa, the first identification of Sarcocystis species in the sika deer by morphological and molecular methods.
Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species
Fresh muscle tissue from six roe deer from Southeastern Norway was examined for sarcocysts andylogenetic analyses based on complete ssu rRNA gene sequences revealed a close relationship between S. gracilis and other canine-transmitted Sarcocystis species, whereas S. oviformis formed a well-supported group with Sarccystis hardangeri of reindeer and Sarcocrystis ovalis of moose.
Muscular sarcocystosis in two arctic foxes (Vulpes lagopus) due to Sarcocystis arctica n. sp.: sarcocyst morphology, molecular characteristics and phylogeny
Sequence comparisons and phylogenetic analyses revealed that the species described from the two arctic foxes was distinct from other named Sarcocystis spp.
Sarcocystis entzerothi n. sp. from the European roe deer (Capreolus capreolus)
Having examined six roe deer hunted in Lithuania, cysts of S. entzerothi and S. silva were identified in four and two animals, respectively, and these two Sarcocystis species could be morphologically differentiated according to the shape of the cysts and the length of protrusions.
Molecular differentiation of Sarcocystis buffalonis and Sarcocystis levinei in water buffaloes (Bubalus bubalis) from Sarcocystis hirsuta and Sarcocystis cruzi in cattle (Bos taurus)
The phylogenetic analyses placed with high support the cox1 sequences of S. buffalonis and S. levinei from water buffaloes with similar sequences deposited in GenBank suggested that the two species were genetically different from S. hirsuta into two monophyletic sister groups.
A Light Microscopic Comparison of The Cysts of Four Species of Sarcocystis Infecting The Domestic Reindeer (Rangifer Tarandus) in Northern Norway
  • B. Gjerde
  • Biology, Medicine
    Acta Veterinaria Scandinavica
  • 1984
Fresh preparations of micro-isolated sarcocysts from skeletal and cardiac muscle of 12 reindeer were examined by light microscopy and 2 previously unnamed Sarcocystis spp.