• Corpus ID: 3956479

Morphological and Molecular Identification of Globodera pallida Associated with Potato in Idaho.

@article{Skantar2007MorphologicalAM,
  title={Morphological and Molecular Identification of Globodera pallida Associated with Potato in Idaho.},
  author={A. M. Skantar and Zafar A. Handoo and Lynn Kay Carta and David J Chitwood},
  journal={Journal of nematology},
  year={2007},
  volume={39 2},
  pages={
          133-44
        }
}
The identity of a newly discovered population of pale potato cyst nematode Globodera pallida associated with potato in eastern Idaho was established by morphological and molecular methods. Morphometrics of cysts and second-stage juveniles were generally within the expected ranges for G. pallida with some variations noted. The Idaho population and paratype material from Epworth, Lincolnshire, England, both showed variations in tail shape, with bluntly rounded to finely pointed tail termini… 

Figures and Tables from this paper

Morphological and molecular characterization of Globodera populations from Oregon and Idaho.
TLDR
An unusual population of cyst nematode was found in soils collected from a Powell Butte, OR field with a cropping history including potato, wheat, other crops, and significant weed presence and exhibited some unique morphological features in some specimens.
Biological characterisation of Globodera pallida from Idaho
TLDR
The overall level of reproduction of the Idaho population on the resistant cultivars was the lowest of all the populations tested, suggesting that the white potato cyst nematode population is not highly virulent.
First report of morphological and molecular characterization of Moroccan populations of Globodera pallida
TLDR
Molecular and morphometric methods were used for identifying and differentiating PCN species in Morocco for the first time and revealed proximity of the Moroccan population to G. pallida species.
PCR-RFLP diagnostic method for identifying Globodera species in Slovenia
TLDR
Sequences of the internal transcribed spacer regions ITS1 and ITS2 of the rDNA gene cluster proved to be useful for identifying nematode species identification and a PCR-RFLP molecular method was used to identify Globodera rostochiensis, G. pallida,G.
Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes Globodera rostochiensis and Globodera pallida (Tylenchida: Heteroderidae) in Algeria
TLDR
The presence of two PCN species, G. pallida and G. rostochiensis, in Algeria is confirmed and additional information on their biogeographic distribution is provided and the distribution of both species throughout various parts of the country was investigated.
Characterization of Globodera rostochiensis (Tylenchida: Heteroderidae) associated with potato in Quebec, Canada
TLDR
The phylogenetic analysis inferred by the sequence of the rDNA ITS region confirmed the identity of the cyst nematode from Quebec, Canada as G. rostochiensis with 100% match to the G. robinsoniensis from Russia, Peru, Japan and UK.
Occurrence and molecular characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia
TLDR
The findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globeodera species, and lead to the development of accurate management strategies for the potato cyst nematodes.
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia
TLDR
The findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globeodera species, and lead to the development of accurate management strategies for the potato cyst nematodes.
Molecular characterization of Canadian populations of potato cyst nematodes, Globodera rostochiensis and G. pallida using ribosomal nuclear RNA and cytochrome b genes
TLDR
The hypothesis that there are possibly several sibling species within G. pallida is confirmed, and sequence analysis revealed that several newly obtained sequences cannot be translated into functional cytb protein, because point indels disrupt the reading frame.
Morphological and molecular identification of potato and cereal cyst nematode isolates from Algeria and their phylogenetic relationships with other populations from distant theirgeographical areas
TLDR
The high divergence among Algerian populations of G. pallida and G. rostochiensis it can be assumed that they were multi-introduced in Algeria, suggests a later or independent introduction of this population into Algeria.
...
...

References

SHOWING 1-10 OF 101 REFERENCES
Identification of Globodera rostochiensis and G. pallida in the Ukraine by PCR
TLDR
It is demonstrated that polymerase chain reaction-mediated amplification of specific regions of the potato cyst nematode genome is not only highly effective as a species diagnostic tool but is also a sensitive method which can be used for taxonomic purposes with cyst collections which vary in age.
First Report of the Pale Cyst Nematode, Globodera pallida, in the United States.
TLDR
The morphology of cysts and second-stage juveniles and molecular analyses established the identity of the species as the pale cyst nematode Globodera pallida, a new country record for G. pallida in the United States.
Molecular approaches to the taxonomic position of Peruvian potato cyst nematodes and gene pool similarities in indigenous and imported populations of Globodera
TLDR
Peruvian potato cyst nematode populations were analysed to assess both their inter- and intraspecific similarities and it was concluded that the primary founders of the European populations may have an origin other than that of the Peruvian populations.
Interspecific rDNA restriction fragment length polymorphism in Globodera species, parasites of Solanaceous plants
TLDR
These results confer a species status ta G. "mexieana" whose genetic identity was unclear and are discussed with regard to previous hybridisation and ta 2D electrophoresis.
Separation of Globodera rostochiensis and G. pallida (Tylenchida: Heteroderidae) using morphology and morphometrics
TLDR
Two Globodera rostochiensis and two G. pallida populations (pure and mixed) were analysed by identification methods based on morphology/morphometrics, suggesting the possibility of designing a computerised expert system.
Intraspecific Variability within Globodera tabacum solanacearum Using Random Amplified Polymorphic DNA.
TLDR
The RAPD markers described here may be useful for the development of specific primers or probes that could improve the identification of TCN populations and facilitate the effective deployment of existing and future resistant cultivars to control these economically important pests.
Intraspecific Variation in Ribosomal DNA in Populations of the Potato Cyst Nematode Globodera pallida.
TLDR
Sequence analysis from six of the populations, together with RFLP analysis of PCR products, shows that there is intraspecific variation in the rDNA of G. pallida.
Variations in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants
The D3 expansion region of the 28S gene and the ITS1-5.8S-ITS2 region of rDNA sequences from Globodera rostochiensis , G. pallida , G. tabacum tabacum , G. tabacum virginiae and G. tabacum
Molecular characterization of a morphologically unusual potato cyst nematode
TLDR
During routine screening of soil samples for potato cyst nematodes, a morphologically variant cyst was detected and clones of individual ITS regions from a single nematode from the variant Cyst displayed either a G. pallida or G. rostochiensis pattern.
Ribosomal DNA comparisons of globodera from two continents.
TLDR
Phylogenetic analysis of the rDNA sequence data indicated that the two potato cyst nematodes, G. pallida and especially G. rostochiensis, are closely related to the Mexican isolates, whereas G. virginiae is relatively dissimilar to the others and more distantly related.
...
...