Morphological Characterization of Femur and Parietal Bone Mineral of Rats at Different Ages

  title={Morphological Characterization of Femur and Parietal Bone Mineral of Rats at Different Ages},
  author={Sergio Bertazzo and Celso Aparecido Bertran and Jos{\'e} Angelo Camilli},
  journal={Key Engineering Materials},
  pages={11 - 14}
Some microscopy studies on the mineral phase of bone have been carried out either utilizing Optical or Scanning Electron Microscopy (SEM), generally around 1000x magnification, to observe the interaction between cells and bone mineral. Other studies have been utilizing Transmission Electron Microscopy (TEM), generally around 1000000x magnification, to observe the fundamental particles that form bone mineral. The literature lacks works that utilize Scanning Electron Microscopy around 10000x… Expand
Size and size distribution of apatite crystals in sauropod fossil bones
Abstract Two complementary techniques, X-ray diffraction and transmission electron microscopy, were used to analyze the size and size distribution of apatite crystallites in subfossil mammal andExpand
Investigation of age related changes to porcine cortical bone using nanoindentation and ash content
Developing bone undergoes significant structural changes at multiple scales. To gain a better understanding of this dynamic, living material, porcine bone properties were analyzed from shortly afterExpand
Crossfibrillar mineral tessellation in normal and Hyp mouse bone as revealed by 3D FIB-SEM microscopy.
A severely altered mineralization pattern invariably leads to abnormal compliance, other aberrant biomechanical properties, and altered remodeling of bone, all of which indubitably lead to macroscopic bone deformities and anomalous mechanical performance in XLH/Hyp. Expand
Local electronic structure and nanolevel hierarchical organization of bone tissue: theory and NEXAFS study.
The NEXAFS analysis has demonstrated the essential hierarchy induced HAP- to-bone red shifts of the Ca and P 2p-to-valence transitions and described the mechanisms ensuring the link of the hierarchical organization with the electronic structure of the mineralized phase in bone. Expand
Biopolymer based structures for biological tissue reconstruction
The thesis entitled Biopolymer based structures for biological tissue reconstruction, structured in four chapters, aims to present the possibility of obtaining biopolymeric structures that can beExpand
Analysis of bone mineral density of different sports and games in university level players
The purpose of the study was to analysis of bone mineral density of different sports and games in university level players. To achieve the purpose of the study, one hundred and twenty men playersExpand
Hierarchy effect on electronic structure and core-to-valence transitions in bone tissue: perspectives in medical nanodiagnostics of mineralized bone
Abstract Electronic structure and core-to-valence transitions in bone tissue are examined in the framework of the morphological 3DSL model that takes into account (i) structural and functionalExpand


TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone.
It was found that crystals were of platelet shape with irregular edges and that there was no substantial difference in crystal length or crystal thickness between normal and osteoporotic trabecular bone. Expand
Shape and size of isolated bone mineralites measured using atomic force microscopy
Using atomic force microscopy, direct three‐dimensional visual evidence is obtained of the size and shape of native protein‐free mineralites isolated from mature bovine bone, providing more accurate inputs to molecular‐scale models aimed at predicting the physiological and mechanical behavior of bone. Expand
Size and Shape of Mineralites in Young Bovine Bone Measured by Atomic Force Microscopy
New AFM data supporting this claim is consistent with findings obtained by electron microscopy and low-angle x-ray and neutron diffraction that mineralites formed within collagen fibrils during initial stages of calcification occur within the hole zone region. Expand
Organization of apatite crystals in human woven bone.
Characteristics of apatite crystals in woven bone provide insight into the spatial and temporal relation between collagen and mineral that is the basis for the structure and organization of the mineral comprising human woven bone. Expand
Chemical and structural characterization of the mineral phase from cortical and trabecular bone.
X-ray diffraction, infrared spectroscopy and chemical investigations have been carried out on the inorganic phases from rat cortical and trabecular bone, finding differences can be related to the different extents of collagen posttranslational modifications exhibited by the two types of bone, in agreement with their different biological functions. Expand
High-resolution AFM imaging of intact and fractured trabecular bone.
Fractured samples, rinsed to remove mineral plates, reveal separated collagen fibrils on the fractured surfaces, providing important insights into the nanostructured architecture of this complex biocomposite. Expand
In vitro growth and differentiation of osteoblast-like cells on hydroxyapatite ceramic granule calcified from red algae.
  • D. Turhani, B. Cvikl, +5 authors R. Ewers
  • Medicine
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  • 2005
Hydxyapatite ceramic bone substitute obtained from calcified red algae support the proliferation and differentiation of human osteoblast-like cells on its surface in vitro and might be suitable for use as scaffolds in tissue engineering strategies in vivo. Expand
Hydrazine-deproteinated bone mineral
Physicochemical data are presented demonstrating that although rat bone crystals more closely resemble synthetic controls made in carbonate-rather than hydroxide-rich media, rat bone apatite cannot be interpreted in terms of known or postulated crystal models in any meaningful fashion. Expand
In vitro behaviour of bone marrow-derived mesenchymal cells cultured on fluorohydroxyapatite-coated substrata with different roughness.
Investigating the behaviour and differentiation of human bone marrow-derived mesenchymal cells cultured on two substrata consisting of fluorohydroxyapatite (FHA)-coated titanium and characterised by different surface roughness indicates that FHAs are cytocompatible materials, which allow the adhesion and growth of osteogenic cells. Expand
Age changes in the crystallinity of bone mineral and in the disorder of its crystal.
It is suggested that lattice imperfections of the first kind more largely contributes to the disorder of bone mineral crystals than those of the second kind. Expand