Morphohistological and flow cytometric analyses of somatic embryogenesis in Trifolium nigrescens Viv.

@article{Konieczny2011MorphohistologicalAF,
  title={Morphohistological and flow cytometric analyses of somatic embryogenesis in Trifolium nigrescens Viv.},
  author={Robert Konieczny and Elwira Śliwińska and Maria Pilarska and Monika Tuleja},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2011},
  volume={109},
  pages={131-141}
}
Microscopy and flow cytometry (FCM) were used to study somatic embryogenesis (SE) from zygotic embryos of Trifolium nigrescens Viv. to determine if there were any relationships between characteristics of somatic embryos (morphology, anatomy, genome size stability) and their regenerability. Embryoids were induced on Murashige and Skoog (MS) medium containing 4 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 2 mg l−1 N6-[2-isopentenyl]-adenine (2iP) either directly from hypocotyls or via an… CONTINUE READING
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