Morphohistological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis

Abstract

Morphohistological analysis and histochemical studies were carried out during the induction and development of Feijoa sellowiana somatic embryos. Zygotic embryos were cultured on LPm medium containing 2,4-dichlorophenoxyacetic acid (20 µM) and glutamine (8 mM). Somatic embryogenesis could be induced from embryogenic cells that originated in meristematic centers or from clusters of cells. The presence of few starch grains and abundant protein bodies was observed in the globular and early torpedo stages, while in torpedo and cotyledonary-stage somatic embryos an enhanced synthesis of starch grains was associated with the accumulation of reserves to be used in the conversion of the embryos to plantlets. Proteins were predominantly observed in protoderm cells, as well as in the meristematic apical region of torpedo and cotyledonary-stage somatic embryos.

DOI: 10.1007/s00709-004-0055-5

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@article{CangahualaInocente2004MorphohistologicalAA, title={Morphohistological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis}, author={Gabriela Claudia Cangahuala-Inocente and Neusa Steiner and Mirentxu Santos and Miguel Pedro Guerra}, journal={Protoplasma}, year={2004}, volume={224}, pages={33-40} }