Morphogenetic clonal growth of kidney epithelial cell line MDCK

@article{Mcateer1987MorphogeneticCG,
  title={Morphogenetic clonal growth of kidney epithelial cell line MDCK},
  author={James A Mcateer and Andrew P. Evan and Kenneth D. Gardner},
  journal={The Anatomical Record},
  year={1987},
  volume={217}
}
MDCK (Madin‐Darby canine kidney) cells were cultured either (1) dispersed within hydrated collagen gel (HCG) or (2) seeded atop a collagen substrate and then immediately overlaid with HCG. Individual cells exhibited clonal growth in three dimensions to form spherical cysts made up of a simple epithelium enclosing a fluid‐filled lumen. The cells of MDCK cysts were polarized with the basolateral surface in contact with the collagen gel and the apical surface bordering the lumen. The… 
MDCK cysts: An in vitro model of epithelial cyst formation and growth
TLDR
This novel method of renal cell culture provides a study system to model the influence of the extracellular matrix on kidney epithelial cell structure and function and offers an in vitro model of general application to the study of epithelial cyst formation and growth.
Role of fibronectin deposition in cystogenesis of Madin-Darby canine kidney cells.
TLDR
The results indicate that the deposition of fibronectin underlying the cyst-lining epithelium serves to prevent apoptosis induced by three-dimensional collagen gel cultures, and hence facilitates cyst growth of MDCK cells.
Role of fibronectin deposition in branching morphogenesis of Madin-Darby canine kidney cells.
TLDR
Results indicate that the deposition of fibronectin underlying the tubule-lining epithelium serves to enhance cell proliferation and migration, and hence facilitates the branching tubulogenesis of MDCK cells.
Type I collagen gel induces Madin-Darby canine kidney cells to become fusiform in shape and lose apical-basal polarity
TLDR
The loss of epithelial polarity and acquisition of mesenchymal cellPolarity and shape by fusiform MDCK cells on type I collagen gel was previously unsuspected and may offer new opportunities for studying cytoplasmic and nuclear mechanisms regulating cell shape and polarity.
In vitro formation and expansion of cysts derived from human renal cortex epithelial cells.
TLDR
It is concluded that the formation and expansion of in vitro cysts derived from solitary human cortex cells depends on the coordinated interplay between cellular proliferation and fluid secretion.
Morphology of Renal Parenchymal Culture in Collagen Gels
TLDR
Histologically an immediate phase of necrosis was followed by regeneration whereby tubules became lined with a confluent epithelium composed of a single layer of flat to cuboidal‐shaped cells sitting on an intact tubular basement membrane (TBM).
Activated extracellular signal-regulated kinases are necessary and sufficient to initiate tubulogenesis in renal tubular MDCK strain I cell cysts.
TLDR
Results suggest that a seminal event in kidney development may be the activation of ERK in the mesonephric duct/ureteric bud cells destined to form the collecting tubules.
Morphogenesis of MDCK cells in a collagen gel matrix culture under stromal adipocyte-epithelial cell interaction.
TLDR
Results indicate that the specific stromal cell type adipocytes cause MDCK cells to organize the well-polarized tubular structures in two different manners according to their direct and indirect interactions, suggesting that adipocytes may be involved in the regulatory mechanism of renal epithelial morphogenesis.
Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation.
TLDR
Because Bcl-2 overexpression averts cyst cavitation, these data clearly indicate that apoptosis is an essential initial event for renal cyst formation.
Age effect of type I collagen on morphogenesis of Mardin-Darby canine kidney cells.
TLDR
Age-related alterations in collagen influence epithelial cell morphogenesis via regulation of cell apoptosis, proliferation, and/or motility through regulation of extracellular matrix.
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