Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein

  title={Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein},
  author={Thilo Winzer and Marcelo Kern and Andrew J. King and Tony R Larson and Roxana Teodor and Samantha L. Donninger and Yi Li and Adam A. Dowle and Jared Cartwright and Rachel Bates and David A. Ashford and Jerry Thomas and Carol Walker and Tim A. Bowser and Ian A Graham},
Morphinan alkaloids from the opium poppy are used for pain relief. The direction of metabolites to morphinan biosynthesis requires isomerization of (S)- to (R)-reticuline. Characterization of high-reticuline poppy mutants revealed a genetic locus, designated STORR [(S)- to (R)-reticuline] that encodes both cytochrome P450 and oxidoreductase modules, the latter belonging to the aldo-keto reductase family. Metabolite analysis of mutant alleles and heterologous expression demonstrate that the P450… CONTINUE READING
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