Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein

@article{Winzer2015MorphinanBI,
  title={Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein},
  author={Thilo Winzer and Marcelo Kern and Andrew J. King and Tony R Larson and Roxana Teodor and Samantha L. Donninger and Yi Li and Adam A. Dowle and Jared Cartwright and Rachel Bates and David A. Ashford and Jerry Thomas and Carol Walker and Tim A. Bowser and Ian A Graham},
  journal={Science},
  year={2015},
  volume={349},
  pages={309-312}
}
Morphinan alkaloids from the opium poppy are used for pain relief. The direction of metabolites to morphinan biosynthesis requires isomerization of (S)- to (R)-reticuline. Characterization of high-reticuline poppy mutants revealed a genetic locus, designated STORR [(S)- to (R)-reticuline] that encodes both cytochrome P450 and oxidoreductase modules, the latter belonging to the aldo-keto reductase family. Metabolite analysis of mutant alleles and heterologous expression demonstrate that the P450… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 57 times over the past 90 days. VIEW TWEETS
15 Citations
33 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 33 references

A microbial biomanufacturing platform for natural and semisynthetic opioids

  • S. Galanie Thodey, C. D. Smolke
  • Nat . Chem . Biol .
  • 2014

Involvement of a natural fusion of a cytochrome P 450 and a hydrolase in mycophenolic acid biosynthesis

  • E. Mnich, K. F. Nielsen, +4 authors K. R. Patil
  • Appl . Environ . Microbiol .
  • 2012

Papaver somniferum 10 - gene cluster for synthesis of the anticancer alkaloid noscapine

  • V. Gazda T. Winzer, Z. He, +9 authors I. A. Graham
  • Science
  • 2012

A bacterial platform for fermentative production of plant alkaloids

  • H. Minami Nakagawa, J. S. Kim, T. Koyanagi, T. Katayama, F. Sato, H. Kumagai
  • Nat . Commun .
  • 2011

CYP 719 B 1 is salutaridine synthase , the C - C phenol - coupling enzyme of morphine biosynthesis in opium poppy

  • M. Rolf Gesell, J. Ziegler, M. L. Díaz Chávez, F. C. Huang, T. M. Kutchan
  • J . Biol . Chem .
  • 2009

Similar Papers

Loading similar papers…