Moonlighting Function of Phytochelatin Synthase1 in Extracellular Defense against Fungal Pathogens1[OPEN]

  title={Moonlighting Function of Phytochelatin Synthase1 in Extracellular Defense against Fungal Pathogens1[OPEN]},
  author={K. H{\'e}maty and M. Lim and C. Cherk and M. Piślewska-Bednarek and Clara S{\'a}nchez-Rodr{\'i}guez and Monica Stein and Ren{\'e} Fuchs and C. Klapprodt and V. Lipka and A. Molina and E. Grill and P. Schulze-Lefert and P. Bednarek and S. Somerville},
  journal={Plant Physiology},
  pages={1920 - 1932}
PCS1 is a moonlighting protein that functions in abiotic stress response and is indispensable for proper extracellular immune responses and pathogen-triggered glucosinolate metabolism. Phytochelatin synthase (PCS) is a key component of heavy metal detoxification in plants. PCS catalyzes both the synthesis of the peptide phytochelatin from glutathione and the degradation of glutathione conjugates via peptidase activity. Here, we describe a role for PCS in disease resistance against plant… Expand
2 Citations
Epidermal chloroplasts are defense-related motile organelles equipped with plant immune components
Evidence is presented that Arabidopsis epidermal chloroplasts control the entry of fungal pathogens, and atypical small chloroplast act as defense-related motile organelles by specifically positioning immune components in the plant epidermis, which is the first site of contact between the plant and pathogens. Expand
The ARP2/3 complex, acting cooperatively with Class I formins, modulates penetration resistance in Arabidopsis against powdery mildew invasion.
The results demonstrate that the ARP2/3 complex and formins, two actin-nucleating systems, act cooperatively and contribute to Arabidopsis penetration resistance to fungal invasion. Expand


The Arabidopsis thaliana Knockout Mutant for Phytochelatin Synthase1 (cad1-3) Is Defective in Callose Deposition, Bacterial Pathogen Defense and Auxin Content, But Shows an Increased Stem Lignification
The enzyme phytochelatin synthase (PCS) has long been studied with regard to its role in metal(loid) detoxification in several organisms, i.e., plants, yeasts, and nematodes. It is in fact widelyExpand
A substrate of the ABC transporter PEN3 stimulates bacterial flagellin (flg22)-induced callose deposition in Arabidopsis thaliana
A specific indole compound is identified that is a substrate for PEN3 and contributes to the plant defense response against microbial pathogens. Expand
Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast
Data demonstrate that PCS genes encode phytochelatin synthases and mediate metal detoxification in eukaryotes and the homologs of this new gene family from Arabidopsis thaliana, Schizosaccharomyces pombe, and interestingly also Caenorhabditis elegans. Expand
Expression of Caenorhabditis elegans PCS in the AtPCS1-deficient Arabidopsis thaliana cad1-3 mutant separates the metal tolerance and non-host resistance functions of phytochelatin synthases.
It is confirmed that plants lacking the functional gene develop a strong cell death phenotype when inoculated with the potato pathogen Phytophthora infestans and found that the C. elegans gene rescues phytochelatin synthesis and cadmium tolerance, but not the defect in non-host resistance, which strongly suggests that the second enzymatic function of AtPCS1, which remains to be defined in detail, is underlying the plant immunity function. Expand
The Function of Glucosinolates and Related Metabolites in Plant Innate Immunity
Experimental evidence indicate that some molecules released during pathogen-triggered glucosinolate metabolism may activate evolutionarily conserved immune responses, possibly through affecting glutathione redox state in the infected cell. Expand
Glutathione Transferase U13 Functions in Pathogen-Triggered Glucosinolate Metabolism1
It is postulated that GSTU13 connects GSH with the pathogen-triggered PEN2 pathway for IG metabolism to deliver metabolites that may have numerous functions in the innate immune system of Arabidopsis. Expand
Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response
It is shown that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens. Expand
A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense
It is proposed that reiterated enzymatic cycles, controlling the generation of toxic molecules and their detoxification, enable the recruitment of glucosinolates in defense responses. Expand
Phytochelatin synthase: of a protease a peptide polymerase made.
  • P. A. Rea
  • Biology, Medicine
  • Physiologia plantarum
  • 2012
The reasoning and experiments behind the conclusion that PC synthases are novel papain-like Cys protease superfamily members are presented and the question of the common need or needs met by PC synthase and their homologs is discussed. Expand
Conservation and clade-specific diversification of pathogen-inducible tryptophan and indole glucosinolate metabolism in Arabidopsis thaliana relatives.
It is concluded that pathogen-inducible IG metabolism in the Brassicaceae is evolutionarily ancient, while other tryptophan-derived branch pathways represent relatively recent manifestations of a plant-pathogen arms race. Expand