Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection.

@article{Hardy1988MonosaccharideAO,
  title={Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection.},
  author={M R Hardy and Ronald R Townsend and Young Choon Lee},
  journal={Analytical biochemistry},
  year={1988},
  volume={170 1},
  pages={54-62}
}
The method of anion exchange chromatography followed by pulsed amperometric detection (AE-PAD; Johnson, D. C., and Polta, T. Z. (1986) Chromatogr. Forum 1, 37-44) has been applied to the compositional analysis of glycoconjugates. Using 22 mM NaOH as a column effluent, underivatized fucose, galactosamine, glucosamine, galactose, glucose, and mannose were readily separated in 15 min at a flow rate of 1 ml/min. The limit of quantification of the monosaccharides was better than 100 pmol (signal to… CONTINUE READING