Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA.

@article{Bujalowski1991MonomersOT,
  title={Monomers of the Escherichia coli SSB-1 mutant protein bind single-stranded DNA.},
  author={W Bujalowski and Timothy M. Lohman},
  journal={Journal of molecular biology},
  year={1991},
  volume={217 1},
  pages={
          63-74
        }
}
The Escherichia coli wild-type single strand binding (SSB) protein is a stable tetramer that binds to single-stranded (ss) DNA in its role in DNA replication, recombination and repair. The ssb-1 mutation, a substitution of tyrosine for histidine-55 within the SSB-1 protein, destabilizes the tetramer with respect to monomers, resulting in a temperature-sensitive defect in a variety of DNA metabolic processes, including replication. Using quenching of the intrinsic SSB-1 tryptophan fluorescence… CONTINUE READING

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