Monomerization as a prerequisite for intramolecular cleavage and shedding of the thyrotropin receptor.

@article{Latif2004MonomerizationAA,
  title={Monomerization as a prerequisite for intramolecular cleavage and shedding of the thyrotropin receptor.},
  author={Rauf Latif and Takao Ando and Terry F Davies},
  journal={Endocrinology},
  year={2004},
  volume={145 12},
  pages={5580-8}
}
The TSH receptor (TSHR) undergoes intramolecular cleavage of the ectodomain yielding a two-subunit structure on the cell surface. Subsequently, the TSHR ectodomains (the alpha- or A-subunits) are shed from the cell surface. In this study we first confirmed TSHR alpha-subunit shedding from tagged-TSHR transfected Chinese hamster ovary cells. We found that TSH exacerbated this phenomenon of TSHR subunit shedding. The 125I-TSH cross-linking technique has been suggested as useful in the assessment… CONTINUE READING