Monomeric site-specific nucleases for genome editing.

  title={Monomeric site-specific nucleases for genome editing.},
  author={Benjamin P. Kleinstiver and Jason M. Wolfs and Tomasz Kolaczyk and Alanna K Roberts and Sherry X Hu and David R. Edgell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={109 21},
Targeted manipulation of complex genomes often requires the introduction of a double-strand break at defined locations by site-specific DNA endonucleases. Here, we describe a monomeric nuclease domain derived from GIY-YIG homing endonucleases for genome-editing applications. Fusion of the GIY-YIG nuclease domain to three-member zinc-finger DNA binding domains generated chimeric GIY-zinc finger endonucleases (GIY-ZFEs). Significantly, the I-TevI-derived fusions (Tev-ZFEs) function in vitro as… CONTINUE READING
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Role of the interdomain linker in distance determination for remote cleavage by homing endonuclease I-TevI

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