Monomeric and Multimeric Blockers of Selectins: Comparison of in vitro and in vivo Activity

@article{Ushakova2005MonomericAM,
  title={Monomeric and Multimeric Blockers of Selectins: Comparison of in vitro and in vivo Activity},
  author={Natalia A. Ushakova and Marina E. Preobrazhenskaya and Michael I Bird and Ros Priest and Andrey V Semenov and Alexey V. Mazurov and Nikolay E Nifantiev and Tatyana V. Pochechueva and Oxana E. Galanina and Nicolai V. Bovin},
  journal={Biochemistry (Moscow)},
  year={2005},
  volume={70},
  pages={432-439}
}
The potency of the oligosaccharides SiaLex, SiaLea, HSO3Lex, and HSO3Lea, their conjugates with polyacrylamide (PAA, 40 kD), and other monomeric and polymeric selectin inhibitors has been compared with that of the polysaccharide fucoidan. The following assay systems were used: 1) a 96-well assay based either on the use of recombinant E-, P-, and L- selectins or an analogous assay with natural P-selectin isolated from human platelets; 2) a platelet-based P-selectin cell assay; and 3) a rat model… CONTINUE READING