Monocyte and neutrophil isolation and migration assays.

  title={Monocyte and neutrophil isolation and migration assays.},
  author={Simon Yona and Richard P.G. Hayhoe and Inbal Avraham-Davidi},
  journal={Current protocols in immunology},
  volume={Chapter 14},
          Unit 14.15
This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of naïve phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow… CONTINUE READING
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