Monocyte/mesangial cell interactions in high-glucose co-cultures.

  title={Monocyte/mesangial cell interactions in high-glucose co-cultures.},
  author={Paolo Men{\`e} and Cristina Caenazzo and Francesco Pugliese and Giulio A. Cinotti and Angela D'Angelo and Spiridione Garbisa and Giovanni Gambaro},
  journal={Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association},
  volume={16 5},
BACKGROUND Monocytes bind to human mesangial cells (HMC) in a co-culture model of leukocyte/ glomerular cell interactions. Since monocytic infiltration has been demonstrated in the early stages of diabetic glomerulopathy, we examined whether co-culture with myelomonocytes of the U937 cell line in media mimicking the diabetic microenvironment modulated phenotype, growth, and extracellular matrix production patterns of HMC. METHODS HMC monolayers grown for 5 days in 5.5 mmol/l (NG) or 30 mmol/l… CONTINUE READING