Monoclonal antibody-defined epitope map of expressed rubella virus protein domains.

@article{Wolinsky1991MonoclonalAE,
  title={Monoclonal antibody-defined epitope map of expressed rubella virus protein domains.},
  author={Jerry S. Wolinsky and Michael McCarthy and O Allen-Cannady and William P. Moore and Rui-liang Jin and Shuyong Cao and Andrew Lovett and Darrin Simmons},
  journal={Journal of virology},
  year={1991},
  volume={65 8},
  pages={3986-94}
}
An expanded library of murine monoclonal antibodies (MAbs) was generated by infecting BALB/C mice with the Therien strain of rubella virus (RV) and selecting secreting hybrids by enzyme-linked immunosorbent assay (ELISA) using purified virion targets. A panel of plasmids containing specified RV cDNA fragments was also constructed by using a variety of strategies with pGE374- and pGE374-derived expression vectors. Hybrid RecA-RV-beta-galactosidase (LacZ)- or RecA-RV-truncated LacZ-containing… CONTINUE READING

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