Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin.

@article{Rdiger1999MonoclonalAI,
  title={Monoclonal antibody ID5: epitope characterization and minimal requirements for the recognition of polyglutamylated alpha- and beta-tubulin.},
  author={Angelika-H. R{\"u}diger and Marc R{\"u}diger and J Wehland and Klaus -Josef Weber},
  journal={European journal of cell biology},
  year={1999},
  volume={78 1},
  pages={15-20}
}
A monoclonal antibody (ID5) raised against the synthetic tetradecapeptide corresponding to the C-terminal region of detyrosinated alpha-tubulin showed an unexpected cross-reactivity with beta-tubulin from pig brain tissue. The specificity and the minimal epitope requirements of ID5 were characterized by competitive enzyme-linked immunosorbent assay (ELISA) and spot blots using a series of synthetic peptides and the natural peptides of beta-tubulin and detyrosinated alpha-tubulin from brain. The… CONTINUE READING

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