Monoclonal antibodies to rabbit skeletal muscle phosphorylase kinase. Probes for studies of subunit function.

Abstract

Monoclonal antibodies to rabbit skeletal muscle phosphorylase kinase were produced by the conventional hybridoma cell technique. 90 out of 600 hybridomas were found to produce phosphorylase kinase binding antibodies from which only five secreted also phosphorylase kinase activity affecting antibodies. Three of them were cloned; two hybridomas resisted all… (More)

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@article{Hessov1985MonoclonalAT, title={Monoclonal antibodies to rabbit skeletal muscle phosphorylase kinase. Probes for studies of subunit function.}, author={Z Hessov{\'a} and Rolf Thieleczek and Marianne Vars{\'a}nyi and Frank W. Falkenberg and L. M. J. Heilmeyer}, journal={The Journal of biological chemistry}, year={1985}, volume={260 18}, pages={10111-7} }