Monoclonal antibodies to purified ricin A-chain: production and properties.

  title={Monoclonal antibodies to purified ricin A-chain: production and properties.},
  author={John E. Leonard and L E Tanney and M. L. Collins and Ivor Royston and Raymond M. Taetle},
  volume={6 2},
Ricin A-chain was purified from native ricin using lactosyl-Sepharose. It was non-toxic to whole cells at a concentration of 1 microM yet nearly as effective as an equimolar concentration of ricin in blocking in vitro protein synthesis. Hybridomas secreting monoclonal antibodies to ricin A-chain were produced using the murine myeloma cell line NS-1. These anti-ricin A-chain antibodies cross-reacted with whole ricin but exhibited little cross-reactivity with purified ricin B-chain. Antibodies… CONTINUE READING

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