Monoclonal antibodies to bovine alpha-crystallin.

  title={Monoclonal antibodies to bovine alpha-crystallin.},
  author={R C Augusteyn and Roland Seifert and L R Tai and Carol H. Thompson},
  journal={Current eye research},
  volume={4 1},
Monoclonal antibodies to bovine alpha-crystallin have been produced using hybridoma technology. Five selected hybridoma clones were maintained as ascites tumours in mice and gram quantities of the antibodies were purified from the ascites fluids by affinity chromatography on alpha m-crystallin covalently bound to Sepharose CL-2B. Preliminary characterization studies suggest that the antibodies are pure and monospecific. The antibodies could be divided into two groups on the basis of their… CONTINUE READING

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