• Biology, Medicine
  • Published in Folia biologica 1993

Monoclonal antibodies to apolipoprotein AI: generation and characterization.

  title={Monoclonal antibodies to apolipoprotein AI: generation and characterization.},
  author={G Falero and Iv{\'a}n Rodr{\'i}guez and Anabel Rojas},
  journal={Folia biologica},
  volume={39 1},
BALB/c mice were immunized with apolipoprotein (apo AI)--high density lipoprotein (HDL) conjugate. By polyethylene glycol-induced fusion of isolated spleen cells with the myeloma cell line P3 X63 Ag8 6.5.3, three different hybridomas were obtained and characterized. Two of them were found to secrete antibodies of the IgG2a subclass, whereas the third produced antibodies of the IgG1 type. Binding capacities to 125I-apo AI and 125I-HDL were higher than 90% in all cases. The isolated antibodies… CONTINUE READING