Monoclonal antibodies specific to haemocytes of black tiger prawn Penaeus monodon.

@article{Winotaphan2005MonoclonalAS,
  title={Monoclonal antibodies specific to haemocytes of black tiger prawn Penaeus monodon.},
  author={Phochit Winotaphan and Paisarn Sithigorngul and Oraporn Muenpol and Siwaporn Longyant and Sombat Rukpratanporn and Parin Chaivisuthangkura and Weerawan Sithigorngul and Amorn Petsom and Piamsak Menasveta},
  journal={Fish & shellfish immunology},
  year={2005},
  volume={18 3},
  pages={189-98}
}
Monoclonal antibodies specific to haemocytes of Penaeus monodon were generated from a mouse immunized with a mixture of SDS-treated and formalin-fixed haemocytes. Hybridoma clones were selected by immunohistochemistry against fixed haemocytes, heart, lymphoid organ, and haemopoietic tissue, and Western blot against haemocyte extract and haemolymph. Sixteen monoclonal antibodies specific to haemocytes were obtained and could be divided into six groups according to their binding capacities to… CONTINUE READING