Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications.

@article{Goh2015MonoclonalAS,
  title={Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications.},
  author={Lucas Y. H. Goh and Jody Hobson-Peters and Natalie A. Prow and Joy Gardner and Helle Bielefeldt-Ohmann and Andreas Suhrbier and Roy A. Hall},
  journal={The Journal of general virology},
  year={2015},
  volume={96 Pt 3},
  pages={
          507-12
        }
}
Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for epidemics of debilitating arthritic disease. The recent outbreak (2004-2014) resulted in an estimated 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. The development of CHIKV-specific diagnostics and research tools is thus highly desirable. Herein we describe the generation and characterization of the first mAbs specific for the capsid protein (CP) of CHIKV. The antibodies recognized isolates… 
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This study mapped the binding sites of a panel of eleven monoclonal antibodies previously generated towards the capsid protein (CP) of CHIKV and provided evidence that the C-terminus of CP is required for authentic antigenic structure of CP.
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A previously uncharacterized nucleolar localization sequence (NoLS) in CHIKV capsid protein is identified, a functional analysis of site-directed mutants of the capsidprotein NoLS is begun, and the effect of the NoLS mutation onCHIKV pathogenesis in vivo and its potential to influence CHikV vaccine design are examined.
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The results provide a simple in vivo model for studying transmission of Chikungunya virus from mosquitoes to mammals and also argue against a resistance barrier to CHIKV infection in adult mice.
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An imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with chikungunya virus proteins, and investigated the contributions of these proteins found that several ESCRT factors are recruited via HGS and areinvolved in viral genome replication and post-translational processing of viral proteins.
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It is found that Chikungunya virus establishes and maintains a persistent infection in joint-associated tissue in part by evading antiviral CD8+ T cell immunity, which suggests immunomodulatory therapies that improve CD8- T cell immune surveillance and clearance of CHikV infection could be a strategy for mitigating chronic CHIKV disease.
Insights into Antibody-Mediated Alphavirus Immunity and Vaccine Development Landscape
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The role of antibody-mediated immunity upon arthritogenic and neurotropic alphavirus infections, and the current research efforts for the development of vaccines as a tool to control future alphvirus outbreaks are reviewed.
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