Monoclonal antibodies recognize at least five epitopes on the SIV Nef protein and identify an in vitro-induced mutation.

@article{Arnold1999MonoclonalAR,
  title={Monoclonal antibodies recognize at least five epitopes on the SIV Nef protein and identify an in vitro-induced mutation.},
  author={Catherine Arnold and Adrian Jenkins and N. W. Almond and E. J. Stott and Kim A Kent},
  journal={AIDS research and human retroviruses},
  year={1999},
  volume={15 12},
  pages={
          1087-97
        }
}
Eleven monoclonal antibodies (MAbs) to SIV Nef were produced and characterized. Five antibody-binding sites on SIV Nef were identified on the basis of the reactivity of the antibodies with recombinant proteins. Two of the five epitopes were defined using overlapping peptides. A further three epitopes could not be defined with peptides but all antibodies reacted in Western blot, suggesting that the epitopes were at least partially conformation dependent. Antibodies in two of the five epitope… CONTINUE READING