Monoclonal antibodies directed against human alpha-thrombin and the thrombin-antithrombin III complex.

@article{Dawes1984MonoclonalAD,
  title={Monoclonal antibodies directed against human alpha-thrombin and the thrombin-antithrombin III complex.},
  author={John. Dawes and Keith James and L R Micklem and Duncan S Pepper and Chris V Prowse},
  journal={Thrombosis research},
  year={1984},
  volume={36 5},
  pages={397-409}
}
Human alpha-thrombin was poorly immunogenic in Balb/c mice. Nevertheless, following fusion of spleen cells from a responding mouse with NS-1 cells, 8 mouse monoclonal antibodies against alpha-thrombin were isolated, and 6 were characterised. Five of these were isotype IgG2a, and one was IgG1. One, EST 1, bound thrombin only minimally, and was directed against a neoantigen on the thrombin-ATIII (T-AT) complex. This antibody also recognised a site on prothrombin, though with much lower affinity… CONTINUE READING

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