Monoclonal antibodies directed against Brucella abortus cell surface antigens.

@article{Bundesen1985MonoclonalAD,
  title={Monoclonal antibodies directed against Brucella abortus cell surface antigens.},
  author={Peter G. Bundesen and David M Wyatt and L E Cottis and Alan S Blake and D A Massingham and William A. Fletcher and G D Street and J. S. Welch and Dennis B. Rylatt},
  journal={Veterinary immunology and immunopathology},
  year={1985},
  volume={8 3},
  pages={245-60}
}
A panel of monoclonal antibodies (MAb) has been raised against Brucella abortus cell surface antigens from mice immunized with either heat/phenol treated or UV killed bacterial suspensions of B. abortus. The hybridomas were screened by either a microagglutination procedure or by an indirect enzyme immunoassay (EIA) on sonicated bacterial preparations. From a large number of MAb generated by various procedures, two distinct types of MAb emerged. The most numerous type was capable of… CONTINUE READING