Monoclonal antibodies as probes for monitoring the denaturation process of bovine beta-lactoglobulin.

@article{Kaminogawa1989MonoclonalAA,
  title={Monoclonal antibodies as probes for monitoring the denaturation process of bovine beta-lactoglobulin.},
  author={Shuichi Kaminogawa and Masato Shimizu and Akio Ametani and Masahiko Hattori and O Ando and Satoshi Hachimura and Yasunori Nakamura and Mamoru Totsuka and Keisuke Yamauchi},
  journal={Biochimica et biophysica acta},
  year={1989},
  volume={998 1},
  pages={
          50-6
        }
}
Five monoclonal antibodies (MAbs) of different idiotypes were produced against bovine beta-lactoglobulin (beta-LG). Among them, MAbs 61B4 and 62A6 reacted preferentially to native beta-LG, while MAbs 21B3 and 31A4 reacted more strongly to the reduced carboxymethylated (denatured) beta-LG than to the native material. These two types of MAb were used to analyze the denaturation process of a beta-LG molecule during heating. The binding affinity of MAbs 21B3 and 31A4 with beta-LG was increased by… CONTINUE READING

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