Monoclonal antibodies against rat Leydig cell surface antigens.

@article{Hedger1986MonoclonalAA,
  title={Monoclonal antibodies against rat Leydig cell surface antigens.},
  author={Mark P Hedger and Edward M. Eddy},
  journal={Biology of reproduction},
  year={1986},
  volume={35 5},
  pages={1309-19}
}
Monoclonal antibodies (MAbs) directed against the Leydig cell surface may be used to identify this cell in testicular preparations. Collagenase-dispersed adult rat interstitial cells were fractionated on Percoll density gradients, and Leydig cell-enriched fractions were used to prepare MAbs. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IIF) on isolated testicular cells and immunocytochemical localization on paraffin sections of adult… CONTINUE READING

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