Monoclonal antibodies against birch pollen allergens: characterization by immunoblotting and use for single-step affinity purification of the major allergen Bet v I.

@article{Jarolim1989MonoclonalAA,
  title={Monoclonal antibodies against birch pollen allergens: characterization by immunoblotting and use for single-step affinity purification of the major allergen Bet v I.},
  author={E Jarolim and M Tejkl and Michal Roh{\'a}c and Gerd Schlerka and Otto Scheiner and Dietrich Kraft and Michael Breitenbach and Helmut Rumpold},
  journal={International archives of allergy and applied immunology},
  year={1989},
  volume={90 1},
  pages={54-60}
}
Two monoclonal antibodies against birch pollen proteins were produced by immunizing BALB/c mice with birch pollen extract. In immunoblotting experiments, antibody BIP 1 reacted with a 17-kilodalton (kD) protein considered to represent the major birch pollen allergen Bet v I. A second monoclonal antibody, BIP 3, reacted with 3 different birch pollen proteins of molecular weights 32, 36 and 68 kD of which the 36- and 68-kD proteins corresponded to minor allergens of birch pollen. Two-dimensional… CONTINUE READING