Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

@inproceedings{Zhao2016MonochromaticMF,
  title={Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions},
  author={Huaying Zhao and Yan Fu and Carla R. Glasser and Eric J Andrade Alba and Mark L Mayer and George H. Patterson and Peter Schuck},
  booktitle={eLife},
  year={2016}
}
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of multiple co-existing complexes. Sedimentation velocity analytical ultracentrifugation equipped with fluorescence detection (FDS-SV) allows the characterization of protein complexes free in solution with high size resolution, at concentrations in… CONTINUE READING