Monitoring transfer of recombinant and nonrecombinant plasmids between Lactococcus lactis strains and members of the human gastrointestinal microbiota in vivo--impact of donor cell number and diet.

@article{Tuohy2002MonitoringTO,
  title={Monitoring transfer of recombinant and nonrecombinant plasmids between Lactococcus lactis strains and members of the human gastrointestinal microbiota in vivo--impact of donor cell number and diet.},
  author={Kieran M Tuohy and Michael P H Davies and Paul C. Rumsby and C J Rumney and Martin R Adams and Ian R. Rowland},
  journal={Journal of applied microbiology},
  year={2002},
  volume={93 6},
  pages={954-64}
}
AIMS The generation of data of real relevance to the purported risks of DNA transfer from food-borne genetically modified microorganisms (GMMOs) using the human biota associated (HBA) rat model. Plasmid transfer between Lactococcus lactis strains and between donor strains and human gut bacteria was monitored. METHODS AND RESULTS Transfer of the recombinant plasmid pCK1 and/or the promiscuous nonrecombinant plasmid pAMbeta1 between L. lactis strains was monitored in vivo in HBA rats. No… CONTINUE READING

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