Monitoring the fate of dietary proteins in rumen fluid using gel electrophoresis.

@article{Spencer1988MonitoringTF,
  title={Monitoring the fate of dietary proteins in rumen fluid using gel electrophoresis.},
  author={Donald Spencer and T J V Higgins and Martin Freer and Hugh Dove and J B Coombe},
  journal={The British journal of nutrition},
  year={1988},
  volume={60 2},
  pages={241-7}
}
1. When fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), strained rumen fluid from sheep fed on pelleted lucerne (Medicago sativa) hay showed no major protein components that stain with Coomassie Blue. This feature made it possible to monitor the fate of individual polypeptides within a protein mixture incubated in rumen fluid in vitro. 2. Extracts from a number of seed meals (sunflower (Helianthus annuus), lupin (Lupinus angustifolius), rape (Brassica napus… CONTINUE READING