Monitoring the developmental status of Trypanosoma brucei gambiense in the tsetse fly by means of PCR analysis of anal and saliva drops.

@article{Ravel2003MonitoringTD,
  title={Monitoring the developmental status of Trypanosoma brucei gambiense in the tsetse fly by means of PCR analysis of anal and saliva drops.},
  author={Sophie Ravel and Pascal Gr{\'e}baut and Dominique Cuisance and G{\'e}rard Cuny},
  journal={Acta tropica},
  year={2003},
  volume={88 2},
  pages={161-5}
}
Teneral Glossina palpalis gambiensis (Diptera: Glossinidae) were infected with a culture of procyclic forms of Trypanosoma brucei gambiense using a single-bloodmeal membrane feeding technique. The infection was monitored by analysing the saliva (mature infection) and anal drop (midgut infection) of each fly at different post-infection times both by microscopic observation and polymerase chain reaction (PCR). Amplification revealed many more positive anal drops than microscopy. The monitoring… CONTINUE READING

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