Monitoring the cellular effects of HMG-CoA reductase inhibitors in vitro and ex vivo.

@article{Cicha2004MonitoringTC,
  title={Monitoring the cellular effects of HMG-CoA reductase inhibitors in vitro and ex vivo.},
  author={Iwona Cicha and Nicole Schneiderhan-Marra and Atilla Yilmaz and Christoph D. Garlichs and Margarete Goppelt-Struebe},
  journal={Arteriosclerosis, thrombosis, and vascular biology},
  year={2004},
  volume={24 11},
  pages={2046-50}
}
OBJECTIVE Inhibition of 3hydroxy3methylglutaryl-coenzyme A (HMG-CoA) reductase by statins and the subsequent reduction in Rho protein isoprenylation inactivates these important signaling molecules. The purpose of this study was to directly monitor statin effects on Rho proteins. METHODS AND RESULTS We used biphasic Triton X-114 system, 1-dimensional isoelectric focusing, and 2D-electrophoresis for the separation of modified and nonmodified Rho proteins. These methods were evaluated in human… CONTINUE READING