Monitoring the T-Cell Receptor Repertoire at Single-Clone Resolution


The adaptive immune system recognizes billions of unique antigens using highly variable T-cell receptors. The alphabeta T-cell receptor repertoire includes an estimated 10(6) different rearranged beta chains per individual. This paper describes a novel micro-array based method that monitors the beta chain repertoire with a resolution of a single T-cell clone. These T-arrays are quantitative and detect T-cell clones at a frequency of less than one T cell in a million, which is 2 logs more sensitive than spectratyping (immunoscope), the current standard in repertoire analysis. Using T-arrays we detected CMV-specific CD4+ and CD8+ T-cell clones that expanded early after viral antigen stimulation in vitro and in vivo. This approach will be useful in monitoring individual T-cell clones in diverse experimental settings, and in identification of T-cell clones associated with infectious disease, autoimmune disease and cancer.

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@article{Bonarius2006MonitoringTT, title={Monitoring the T-Cell Receptor Repertoire at Single-Clone Resolution}, author={Hendrik P.J. Bonarius and Frank Baas and Ester B. M. Remmerswaal and Ren{\'e} A W van Lier and Ineke J. M. ten Berge and Paul Peter Tak and Niek de Vries}, journal={PLoS ONE}, year={2006}, volume={1}, pages={395 - 402} }