Monitoring system for the biocontrol agent Pichia anomala strain K using quantitative competitive PCR-ELOSA

@inproceedings{Pujol2004MonitoringSF,
  title={Monitoring system for the biocontrol agent Pichia anomala strain K using quantitative competitive PCR-ELOSA},
  author={Merardo Pujol and Dirk De Clercq and S. Cognet and Philippe Lepoivre and Mohamed Ha{\"i}ssam Jijakli},
  year={2004}
}
A quantitative-competitive PCR (QC-PCR) method with enzyme-linked oligosorbent assay (ELOSA) was developed to monitor Pichia anomala strain K, a biocontrol agent against postharvest diseases on apples. The procedure involved: (i) extraction of strain K DNA; (ii) coamplification of a constant amount of the extracted DNA (containing a strain K DNA marker of 262 base pairs (bp) specifically amplified with SCAR primers K1 and K2) with an internal standard (IS) titration; and (iii) differential… CONTINUE READING
4 Extracted Citations
24 Extracted References
Similar Papers

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 24 references

Development of a SCAR marker and a semi-selective medium for specific quantification of Pichia anomala strain

  • D De Clercq, S Cognet, M Pujol, P Lepoivre, MH Jijakli
  • K on apple surface. Postharvest Biology and…
  • 2003
Highly Influential
4 Excerpts

Pre- and post-harvest practical application of Pichia anomala strain K, β-1,3-glucans and calcium chloride on apples: two years of monitoring and efficacy against post-harvest diseases

  • MH Jijakli, D De Clercq, C Dickburt, P Lepoivre
  • IOBC/ WPRS Bulletin
  • 2002
Highly Influential
5 Excerpts

Development of a SCAR marker and a semi - selective medium for specific quantification of Pichia anomala strain K on apple surface

  • D DeClercq, S Cognet, M Pujol, P Lepoivre, MH Jijakli
  • Postharvest Biology and Technology
  • 2003

Similar Papers

Loading similar papers…