Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope☆

@inproceedings{FernndezAlfonso2014MonitoringSA,
  title={Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope☆},
  author={Tom{\'a}s Fern{\'a}ndez-Alfonso and Kalyan S. Nadella and Maria Florencia Iacaruso and Bruno Pichler and Hana Ro{\vs} and P. Kirkby and Robin Angus Silver},
  booktitle={Journal of Neuroscience Methods},
  year={2014}
}
BACKGROUND Two-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded… CONTINUE READING
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