Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy.

@article{Ktting2005MonitoringPI,
  title={Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy.},
  author={Carsten K{\"o}tting and Klaus Gerwert},
  journal={Methods in molecular biology},
  year={2005},
  volume={305},
  pages={
          261-86
        }
}
Time-resolved FTIR difference spectroscopy is a valuable tool to monitor the dynamics of protein-ligand interactions, which selects out of the background absorbance of the whole sample the absorbance bands of the protein groups and of the ligands, which are involved in the protein reaction. The absorbance changes can be monitored with time-resolutions down to nanoseconds and followed then over nine orders of time up to seconds even in membrane proteins with the size of 100,000 Dalton. Here, we… CONTINUE READING