Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.

@article{Gelder2008MonitoringPP,
  title={Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.},
  author={Joke De Gelder and Diana F M Willemse-Erix and Maarten J Scholtes and Jorge Isidro Aranda Sanchez and Kees Maquelin and Peter Vandenabeele and Patrick De Boever and Gerwin J. Puppels and Luc Moens and Paul De Vos},
  journal={Analytical chemistry},
  year={2008},
  volume={80 6},
  pages={2155-60}
}
This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial… CONTINUE READING

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