Monitoring peptide tyrosine nitration by spectroscopic methods.

@article{Niederhafner2020MonitoringPT,
  title={Monitoring peptide tyrosine nitration by spectroscopic methods.},
  author={P. Niederhafner and M. {\vS}afař{\'i}k and Jitka Neburkov{\'a} and T. Keiderling and P. Bouř and Jaroslav {\vS}ebest{\'i}k},
  journal={Amino acids},
  year={2020}
}
Oxidative stress can lead to various derivatives of the tyrosine residue in peptides and proteins. A typical product is 3-nitro-L-tyrosine residue (Nit), which can affect protein behavior during neurodegenerative processes, such as those associated with Alzheimer's and Parkinson's diseases. Surface enhanced Raman spectroscopy (SERS) is a technique with potential for detecting peptides and their metabolic products at very low concentrations. To explore the applicability to Nit, we use SERS to… Expand
Post-translational modifications (PTM): analytical approaches, signaling, physiology and pathophysiology—part I
TLDR
The examples and the low-molecular-mass bioactive AGEs indicate that PTM does not only modify proteins, but may also serve as a source for biologically active metabolites and biomarkers, such as HbA1c, one of the earliest indices of diabetic control. Expand
Chiral detection by induced surface-enhanced Raman optical activity.
TLDR
A measurement protocol based on silver colloids and aromatic linkers where chiral acids could be detected in concentrations of about 10-5 M and explained by binding and self-assembly of the linkers into chiral aggregates on the silver surface. Expand

References

SHOWING 1-10 OF 123 REFERENCES
Identification and relative quantification of tyrosine nitration in a model peptide using two-dimensional infrared spectroscopy.
TLDR
It is concluded that EVV 2DIR spectroscopy is able to provide detailed spectroscopic information on peptide side-chain modifications and to detect nitration levels down to 1% and it is proposed that lower nitration Levels could be detected by introducing a resonant Raman probe step to increase the detection sensitivity of the technique. Expand
Proteomic approaches to analyze protein tyrosine nitration.
TLDR
Methods involving multidimensional chromatography, precursor ion scanning, and/or chemical derivatization have emerged for both identification and quantitation of protein nitration sites and a few have successfully detected endogenous 3NY modifications from biological samples. Expand
Nitration of internal tyrosine of cytochrome c probed by resonance Raman scattering.
TLDR
This initial study indicates that resonance Raman scattering does have unique advantages as an in situ probe of the local structure of nitrated tyrosine residues. Expand
Protein tyrosine nitration: selectivity, physicochemical and biological consequences, denitration, and proteomics methods for the identification of tyrosine-nitrated proteins.
TLDR
The main nitration reactions and elucidate why nitration is not a random chemical process are reviewed and the possibility of an in vivo denitration process is indicated. Expand
Protein tyrosine nitration: biochemical mechanisms and structural basis of functional effects.
  • R. Radi
  • Medicine, Chemistry
  • Accounts of chemical research
  • 2013
TLDR
Recent structural biology data of two pure and homogenously nitrated mitochondrial proteins are analyzed to illustrate regioselectivity and structural effects of tyrosine nitration and subsequent impact in protein loss- or even gain-of-function. Expand
Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples.
TLDR
The immunoaffinity-MS method was applied to quantify the changes in protein tyrosine nitration in mouse lungs after intranasal poly(I:C) treatment and quantified 237 NT sites, indicating that the immuno affinities method can be used for quantitative analysis of protein nitrotyrosines in complex samples. Expand
Mass spectrometry and 3-nitrotyrosine: strategies, controversies, and our current perspective.
TLDR
It is shown that the application of sophisticated mass spectrometric techniques is advantageous if not essential, but that this alone is by no means a guarantee of accurate findings. Expand
Proteomic detection of nitroproteins as potential biomarkers for cardiovascular disease.
TLDR
In vitro and in vivo aspects of redox-proteomics of specific nitroproteins that could be relevant to biomarker analysis and understanding of cardiovascular disease mechanism will be discussed within this review. Expand
3-Nitrotyrosine and related derivatives in proteins: precursors, radical intermediates and impact in function.
TLDR
While NO2Tyr is a well-established stable oxidative stress biomarker and a good predictor of disease progression, its role as a pathogenic mediator has been laboriously defined for just a small number of nitrated proteins and awaits further studies. Expand
Site-specific nitration and oxidative dityrosine bridging of the tau protein by peroxynitrite: implications for Alzheimer's disease.
TLDR
The results suggest that the nitrative and oxidative roles of ONOO- differentially affect tau polymerization and that ONOO(-)-mediated cross-linking could facilitate tau aggregation in AD. Expand
...
1
2
3
4
5
...