Monitoring of cobalt(II) uptake and transformation in cells of the plant-associated soil bacterium Azospirillum brasilense using emission Mössbauer spectroscopy

@article{Kamnev2004MonitoringOC,
  title={Monitoring of cobalt(II) uptake and transformation in cells of the plant-associated soil bacterium Azospirillum brasilense using emission M{\"o}ssbauer spectroscopy},
  author={Alexander A. Kamnev and Lyudmila P. Antonyuk and Leonid A. Kulikov and Yury D. Perfiliev},
  journal={Biometals},
  year={2004},
  volume={17},
  pages={457-466}
}
Interaction of cobalt(II) at micromolar concentrations with live cells of the plant-growth-promoting rhizobacterium Azospirillum brasilense (strain Sp245) and further transformations of the metal cation were monitored using 57Co emission Mössbauer spectroscopy (EMS). Cell suspensions of the bacterial culture (2.4×108 cells ml−1) were doped with radioactive 57CoCl2 (1 mCi; final concentration 2×10−6 M 57Co2+), kept under physiological conditions for various periods of time (from 2 min up to 1… CONTINUE READING

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